Features

General features:

  • create figure according to exact size necessary for desired journal in inches

  • automatically perfectly aligns plots and images in figure panels

  • Display illustrations as png file; or as powerpoint file to unify font size

  • …

Features to display images:

  • Provide images as single Hyperstack or single images

  • Define which timepoints, channels etc. to display

  • Position of images is automatically determined… but can be customized as well

  • Contrast set in ImageJ is automatically extracted from Hyperstack

  • Add annotations (star, asterisk, arrow, lines,text), also using GUI

  • Define regions to be zoomed in, crop images, also using GUI

  • Add timestamp, scale bar, channel names

  • Annotate outside of image based on dimension (e.g. Annotate all timepoints on the outside)

  • …

Features to display quantifications:

  • Provide .csv file

  • Plot data automatically in the allocated space (Boxplot with overlaying data points)

  • Calculate significance and annotate in image (Largely based on Statannot from Marc Weber)

  • Show statistics (e.g. Mean, median, SEM, etc) for each data group (Largely based on Statannot from Marc Weber)

  • Show sorted list of representative cells (closest to average)

  • …

Features to create Movie

  • Movies can be generated as full-fledged figure objects

  • Images can be supplied as ImageJ Hyperstack with the “frames” attribute being animated

  • Lineplots of quantifications (.csv file) can be animated based on the x-attribute

  • Add title pages to movies with introductory text

  • Precisely define movie quality (resolution and bitrate)

  • …